PrimePCR™ SYBR® Green Assay: Rnaset2b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCIP0062619

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0061470
Assay Design:   Intron-spanning
Chromosome Location:   17:6996489-6998008question
Amplicon Length:   101
Splice Variants Targeted:   ENSMUST00000043503 ENSMUST00000167352 ENSMUST00000089119 ENSMUST00000179728 ENSMUST00000097420 ENSMUST00000058383 ENSMUST00000053065 ENSMUST00000180063 ENSMUST00000099400

Gene Information

Description Not Available

Gene Symbol:   Rnaset2b
Gene Name:   Ribonuclease T2B
Aliases:   0610007O07Rik, 4833423A10Rik, 4930532K22Rik, C79750, RNASE6PL, Rnaset2
RefSeq:   NC_000083.6 NT_039636.8
Ensembl:   ENSMUSG00000094724
Entrez:   68195
UniGene:   Mm.181237
Chromosome Mapping:   17|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.970000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download