PrimePCR™ SYBR® Green Assay: Sly, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCIP0062584

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0061435
Assay Design:   Intron-spanning
Chromosome Location:   Y:79326452-79334202question
Amplicon Length:   96
Splice Variants Targeted:   ENSMUST00000178112 ENSMUST00000179076 ENSMUST00000178403 ENSMUST00000177842 ENSMUST00000178380 ENSMUST00000179884 ENSMUST00000180170 ENSMUST00000180332 ENSMUST00000177713 ENSMUST00000180058 ENSMUST00000179551 ENSMUST00000178639 ENSMUST00000179672 ENSMUST00000179330 ENSMUST00000180133 ENSMUST00000179631 ENSMUST00000180249 ENSMUST00000177834 ENSMUST00000178213 ENSMUST00000179024 ENSMUST00000179480 ENSMUST00000179336 ENSMUST00000178751 ENSMUST00000177626 ENSMUST00000178063 ENSMUST00000179811 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Sly
Gene Name:   Sycp3 like Y-linked
Aliases:   Not Available
RefSeq:   NC_000087.7 NT_187050.1
Ensembl:   ENSMUSG00000094746
Entrez:   100042417
UniGene:   Mm.304354
Chromosome Mapping:   Y|Y

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.450000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download