PrimePCR™ SYBR® Green Assay: Sprr2a1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0044097

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Sprr2a1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Sprr2a1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0044059
Assay Design:   Intron-spanning
Chromosome Location:   3:92215894-92219152question
Amplicon Length:   119
Splice Variants Targeted:   ENSMUST00000133843 ENSMUST00000035565 ENSMUST00000032263 ENSMUST00000129404 ENSMUST00000105426 ENSMUST00000131321 ENSMUST00000126920 ENSMUST00000147189 ENSMUST00000105424 ENSMUST00000092420 ENSMUST00000105429 ENSMUST00000064562 ENSMUST00000025585 ENSMUST00000169350 ENSMUST00000043676 ENSMUST00000021420 ENSMUST00000110177 ENSMUST00000003908 ENSMUST00000106485 ENSMUST00000074449 ENSMUST00000071805 ENSMUST00000144386 ENSMUST00000153799 ENSMUST00000034086 ENSMUST00000169179 ENSMUST00000110661 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Sprr2a1
Gene Name:   Small proline-rich protein 2A1
Aliases:   Sprr2a
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000078664
Entrez:   20755
Chromosome Mapping:   3 F1|3 45.2 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 38.670000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download