PrimePCR™ SYBR® Green Assay: Oog1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043982

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Oog1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Oog1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0041766
Assay Design:   Intron-spanning
Chromosome Location:   12:87602693-87606037question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000093389 ENSMUST00000160953 ENSMUST00000159078 ENSMUST00000170109 ENSMUST00000062202 ENSMUST00000163688 ENSMUST00000002883 ENSMUST00000057228 ENSMUST00000179619 ENSMUST00000093271 ENSMUST00000082197 ENSMUST00000101377 ENSMUST00000101376 ENSMUST00000038753 ENSMUST00000095509 ENSMUST00000181843 ENSMUST00000180706 ENSMUST00000181394 ENSMUST00000110145 ENSMUST00000181326 ENSMUST00000061277 ENSMUST00000073532 ENSMUST00000085144 ENSMUST00000105770 ENSMUST00000105754 ENSMUST00000105753 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Oog1
Gene Name:   Oogenesin 1
Aliases:   Oog, c-1
RefSeq:   NC_000078.6 NT_039551.8
Ensembl:   ENSMUSG00000096576
Entrez:   193322
Chromosome Mapping:   12 D2|12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 40.420000
Efficiency 83

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download