PrimePCR™ SYBR® Green Assay: AC134575.1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity; Same primer pair as used in probe assay qMmuCIP0043895

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AC134575.1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AC134575.1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0041679
Assay Design:   Intron-spanning
Chromosome Location:   14:69358937-69361710question
Amplicon Length:   94
Splice Variants Targeted:   ENSMUST00000040912 ENSMUST00000035695 ENSMUST00000164415 ENSMUST00000046245 ENSMUST00000110559 ENSMUST00000149579 ENSMUST00000098057 ENSMUST00000064831 ENSMUST00000168356 ENSMUST00000113215 ENSMUST00000004686 ENSMUST00000084535 ENSMUST00000071044 ENSMUST00000171807 ENSMUST00000163761 ENSMUST00000177371 ENSMUST00000051386 ENSMUST00000015791 ENSMUST00000033506 ENSMUST00000026208 ENSMUST00000156159 ENSMUST00000047526 ENSMUST00000002044

Gene Information

Description Not Available

Gene Symbol:   AC134575.1
Gene Name:   Ectonucleoside triphosphate diphosphohydrolase 4
Aliases:   1200014F22Rik, 4930513O14Rik, A430102M06Rik, AI414893, Lysal1, mKIAA0392
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000095463
Entrez:   67464
Chromosome Mapping:   14 D2|14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.680000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download