PrimePCR™ SYBR® Green Assay: Hsd3b4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043874

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0041658
Assay Design:   Intron-spanning
Chromosome Location:   3:98500228-98503860question
Amplicon Length:   111
Splice Variants Targeted:   ENSMUST00000075245 ENSMUST00000020112 ENSMUST00000099492 ENSMUST00000042057 ENSMUST00000069648 ENSMUST00000084524 ENSMUST00000086023 ENSMUST00000051849 ENSMUST00000152782 ENSMUST00000126532 ENSMUST00000179453 ENSMUST00000028781 ENSMUST00000038859 ENSMUST00000105690 ENSMUST00000122059 ENSMUST00000118704 ENSMUST00000105688 ENSMUST00000177654 ENSMUST00000080953 ENSMUST00000159386 ENSMUST00000034820 ENSMUST00000162583 ENSMUST00000160882 ENSMUST00000162973 ENSMUST00000159050 ENSMUST00000161207 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Hsd3b4
Gene Name:   Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 4
Aliases:   Not Available
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000095143
Entrez:   15495
UniGene:   Mm.475664
Chromosome Mapping:   3 F2.2|3 49.1 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 37.600000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download