PrimePCR™ SYBR® Green Assay: Dynlt1a, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043757

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0041541
Assay Design:   Intron-spanning
Chromosome Location:   17:6311726-6317442question
Amplicon Length:   188
Splice Variants Targeted:   ENSMUST00000169415 ENSMUST00000179569 ENSMUST00000092966 ENSMUST00000179554 ENSMUST00000034982 ENSMUST00000179416 ENSMUST00000165768 ENSMUST00000179065 ENSMUST00000165120

Gene Information

Description Not Available

Gene Symbol:   Dynlt1a
Gene Name:   Dynein light chain Tctex-type 1A
Aliases:   Not Available
RefSeq:   NC_000083.6 NT_039636.8
Ensembl:   ENSMUSG00000092074
Entrez:   100310872
UniGene:   Mm.428775
Chromosome Mapping:   17|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 39.550000
Efficiency 87

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download