PrimePCR™ SYBR® Green Assay: Slc35f4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043661

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Slc35f4, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Slc35f4, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0041445
Assay Design:   Intron-spanning
Chromosome Location:   14:49306230-49314026question
Amplicon Length:   111
Splice Variants Targeted:   ENSMUST00000037120 ENSMUST00000067218 ENSMUST00000115470 ENSMUST00000115028 ENSMUST00000069345 ENSMUST00000023846 ENSMUST00000174581 ENSMUST00000004143 ENSMUST00000107358 ENSMUST00000004145 ENSMUST00000107356 ENSMUST00000107357 ENSMUST00000102638 ENSMUST00000102637 ENSMUST00000078912 ENSMUST00000173494

Gene Information

Description Not Available

Gene Symbol:   Slc35f4
Gene Name:   Solute carrier family 35, member F4
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000079246
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 37.880000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download