PrimePCR™ SYBR® Green Assay: Col22a1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043654

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0041438
Assay Design:   Intron-spanning
Chromosome Location:   15:71937587-71951137question
Amplicon Length:   199
Splice Variants Targeted:   ENSMUST00000166877 ENSMUST00000106612 ENSMUST00000072804 ENSMUST00000169129 ENSMUST00000174409 ENSMUST00000108580 ENSMUST00000048680 ENSMUST00000042944 ENSMUST00000115345 ENSMUST00000081893 ENSMUST00000162424 ENSMUST00000161381 ENSMUST00000034654 ENSMUST00000003870 ENSMUST00000025998 ENSMUST00000111737 ENSMUST00000147152 ENSMUST00000115537 ENSMUST00000167360 ENSMUST00000047970 ENSMUST00000095261 ENSMUST00000138127 ENSMUST00000078626 ENSMUST00000102521 ENSMUST00000047488 ENSMUST00000075164 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Col22a1
Gene Name:   Collagen, type XXII, alpha 1
Aliases:   2310067L16Rik, C80743
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000079022
Entrez:   69700
UniGene:   Not Available
Chromosome Mapping:   15 D3|15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 37.250000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download