PrimePCR™ SYBR® Green Assay: Gal3st4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043619

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Gal3st4, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Gal3st4, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0041403
Assay Design:   Intron-spanning
Chromosome Location:   5:138266229-138270836question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000045726 ENSMUST00000162812 ENSMUST00000161273 ENSMUST00000161938 ENSMUST00000086123 ENSMUST00000062153 ENSMUST00000084414 ENSMUST00000035565 ENSMUST00000095427 ENSMUST00000118366 ENSMUST00000108362 ENSMUST00000108364 ENSMUST00000108363 ENSMUST00000011895 ENSMUST00000172269 ENSMUST00000134663 ENSMUST00000078271 ENSMUST00000033939 ENSMUST00000063401 ENSMUST00000030138 ENSMUST00000111752 ENSMUST00000086317 ENSMUST00000168288 ENSMUST00000163821 ENSMUST00000002079 ENSMUST00000114430 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Gal3st4
Gene Name:   Galactose-3-O-sulfotransferase 4
Aliases:   1500031A01Rik
RefSeq:   NC_000071.6 NT_039315.8
Ensembl:   ENSMUSG00000075593
Entrez:   330217
Chromosome Mapping:   5 G2|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 40.840000
Efficiency 82

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download