PrimePCR™ SYBR® Green Assay: DXBay18, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043513

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0041297
Assay Design:   Intron-spanning
Chromosome Location:   X:73139582-73141640question
Amplicon Length:   194
Splice Variants Targeted:   ENSMUST00000030676 ENSMUST00000136474 ENSMUST00000025520 ENSMUST00000105902 ENSMUST00000105900 ENSMUST00000069620 ENSMUST00000165133 ENSMUST00000002291 ENSMUST00000064454 ENSMUST00000169834 ENSMUST00000088459 ENSMUST00000179117 ENSMUST00000080324 ENSMUST00000118022 ENSMUST00000067417 ENSMUST00000121312 ENSMUST00000034689 ENSMUST00000088987 ENSMUST00000161650 ENSMUST00000161220 ENSMUST00000160780 ENSMUST00000119118 ENSMUST00000088970 ENSMUST00000109155 ENSMUST00000063710 ENSMUST00000075483 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   DXBay18
Gene Name:   DNA segment, Chr X, Baylor 18
Aliases:   Not Available
RefSeq:   NC_000086.7 NT_039706.8
Ensembl:   ENSMUSG00000071745
Entrez:   574405
UniGene:   Mm.336093
Chromosome Mapping:   X A7.3|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 37.120000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download