PrimePCR™ SYBR® Green Assay: Xlr4b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity; Same primer pair as used in probe assay qMmuCIP0043431

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Xlr4b, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Xlr4b, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0041214
Assay Design:   Intron-spanning
Chromosome Location:   X:73217812-73220015question
Amplicon Length:   141
Splice Variants Targeted:   ENSMUST00000078775 ENSMUST00000139191 ENSMUST00000156188 ENSMUST00000069077 ENSMUST00000069103 ENSMUST00000114506 ENSMUST00000081827 ENSMUST00000068551 ENSMUST00000114531 ENSMUST00000114530 ENSMUST00000103198 ENSMUST00000146454 ENSMUST00000071539 ENSMUST00000042657 ENSMUST00000106633 ENSMUST00000125890 ENSMUST00000085050

Gene Information

Description Not Available

Gene Symbol:   Xlr4b
Gene Name:   X-linked lymphocyte-regulated 4B
Aliases:   Xlr4
RefSeq:   NC_000086.7 NT_039706.8 NT_039747.4
Ensembl:   ENSMUSG00000067768
Entrez:   27083
Chromosome Mapping:   X A7.3|X 29.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 37.010000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download