PrimePCR™ SYBR® Green Assay: Snrpg, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043245

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0041028
Assay Design:   Intron-spanning
Chromosome Location:   6:86371583-86378781question
Amplicon Length:   199
Splice Variants Targeted:   ENSMUST00000179801 ENSMUST00000175916 ENSMUST00000010319 ENSMUST00000177288 ENSMUST00000024866 ENSMUST00000053575 ENSMUST00000067048 ENSMUST00000163139 ENSMUST00000049845 ENSMUST00000112366 ENSMUST00000077489 ENSMUST00000112371 ENSMUST00000145934 ENSMUST00000092892 ENSMUST00000001127 ENSMUST00000054245 ENSMUST00000092852 ENSMUST00000089558

Gene Information

Description Not Available

Gene Symbol:   Snrpg
Gene Name:   Small nuclear ribonucleoprotein polypeptide G
Aliases:   2810024K17Rik, AL022803, SMG, sm-G, snRNP-G
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000057278
Entrez:   68011
UniGene:   Mm.276802
Chromosome Mapping:   6|6 B1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.340000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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