PrimePCR™ SYBR® Green Assay: Klk1b26, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043212

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0040995
Assay Design:   Intron-spanning
Chromosome Location:   7:44012678-44014893question
Amplicon Length:   183
Splice Variants Targeted:   ENSMUST00000062555 ENSMUST00000161254 ENSMUST00000093427 ENSMUST00000076251 ENSMUST00000085427 ENSMUST00000062829 ENSMUST00000171987 ENSMUST00000048945 ENSMUST00000005933 ENSMUST00000100737 ENSMUST00000169350 ENSMUST00000043676 ENSMUST00000097177 ENSMUST00000030490 ENSMUST00000167242 ENSMUST00000022696 ENSMUST00000076226 ENSMUST00000164095 ENSMUST00000110319 ENSMUST00000053068 ENSMUST00000122036 ENSMUST00000077498 ENSMUST00000172582 ENSMUST00000119082 ENSMUST00000067530 ENSMUST00000026922 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Klk1b26
Gene Name:   Kallikrein 1-related petidase b26
Aliases:   Egfbp2, Klk26, PRECE-2, mGK-26, mGK26
RefSeq:   NC_000073.6 NT_187035.1
Ensembl:   ENSMUSG00000053719
Entrez:   16618
Chromosome Mapping:   7 B4|7 23.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 37.360000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download