PrimePCR™ SYBR® Green Assay: Chmp6, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043054

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0040837
Assay Design:   Intron-spanning
Chromosome Location:   11:119915686-119916724question
Amplicon Length:   135
Splice Variants Targeted:   ENSMUST00000048977 ENSMUST00000172532 ENSMUST00000159348 ENSMUST00000160561 ENSMUST00000132197 ENSMUST00000026434 ENSMUST00000148232 ENSMUST00000067924

Gene Information

Description Not Available

Gene Symbol:   Chmp6
Gene Name:   Charged multivesicular body protein 6
Aliases:   2400004G01Rik
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000025371
Entrez:   208092
UniGene:   Mm.306673
Chromosome Mapping:   11 E2|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 37.550000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download