PrimePCR™ SYBR® Green Assay: Hmgb3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043015

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Hmgb3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Hmgb3, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0040798
Assay Design:   Intron-spanning
Chromosome Location:   X:71558165-71559136question
Amplicon Length:   192
Splice Variants Targeted:   ENSMUST00000118199 ENSMUST00000030563 ENSMUST00000045896 ENSMUST00000043503 ENSMUST00000132670 ENSMUST00000020123 ENSMUST00000054786 ENSMUST00000059270 ENSMUST00000072699 ENSMUST00000114582 ENSMUST00000015361 ENSMUST00000088874 ENSMUST00000123100 ENSMUST00000078030 ENSMUST00000015877 ENSMUST00000044766 ENSMUST00000119422 ENSMUST00000043707 ENSMUST00000168302 ENSMUST00000027277 ENSMUST00000063444 ENSMUST00000090791 ENSMUST00000050897 ENSMUST00000045713 ENSMUST00000092849 ENSMUST00000080461 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Hmgb3
Gene Name:   High mobility group box 3
Aliases:   Hmg2a, Hmg4
RefSeq:   NC_000086.7 NT_039706.8
Ensembl:   ENSMUSG00000015217
Entrez:   15354
Chromosome Mapping:   X|X A1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 37.370000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download