PrimePCR™ SYBR® Green Assay: B2m, Mouse

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0042770

List Price:  
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0040553
Assay Design:   intron_spanning
Chromosome Location:   2:122147704-122150913question
Amplicon Length:   112
Splice Variants Targeted:   ENSMUST00000102476

Gene Information

Description Not Available

Gene Symbol:   B2m
Gene Name:   Beta-2 microglobulin
Aliases:   Ly-m11, beta2-m, beta2m
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000060802
Entrez:   12010
UniGene:   Mm.163
Chromosome Mapping   2 F1-F3|2 69.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.360000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev C Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev D Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.