PrimePCR™ SYBR® Green Assay: Pqlc1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0042764

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Pqlc1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Pqlc1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0040547
Assay Design:   Intron-spanning
Chromosome Location:   18:80257042-80263491question
Amplicon Length:   119
Splice Variants Targeted:   ENSMUST00000131780 ENSMUST00000157056 ENSMUST00000123750 ENSMUST00000144468 ENSMUST00000129043 ENSMUST00000140594 ENSMUST00000070135 ENSMUST00000091798

Gene Information

Description Not Available

Gene Symbol:   Pqlc1
Gene Name:   PQ loop repeat containing 1
Aliases:   2310009N05Rik, 4933425L21Rik, 5730564E11Rik, C78974
RefSeq:   NC_000084.6 NT_039678.8
Ensembl:   ENSMUSG00000034006
Entrez:   66943
Chromosome Mapping:   18 E3|18

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.080000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download