PrimePCR™ SYBR® Green Assay: Tbp, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0042759

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Assay Information

Technology:   qPCR
Assay Type   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0040542
Assay Design:   Intron-spanning
Chromosome Location:   17:15503049-15504325question
Amplicon Length:   102
Splice Variants Targeted:   ENSMUST00000159197 ENSMUST00000162505 ENSMUST00000014911 ENSMUST00000147081 ENSMUST00000118001 ENSMUST00000143924 ENSMUST00000119879 ENSMUST00000155051 ENSMUST00000117593

Gene Information

Description Not Available

Gene Symbol:   Tbp
Gene Name:   TATA box binding protein
Aliases:   GTF2D1, Gtf2d, SCA17, TFIID
RefSeq:   NC_000083.6 NT_039649.8
Ensembl:   ENSMUSG00000014767
Entrez:   21374
UniGene:   Mm.244820
Chromosome Mapping   17 A2|17 8.25 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.740000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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