PrimePCR™ SYBR® Green Assay: Rnf32, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0042327

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0040109
Assay Design:   Intron-spanning
Chromosome Location:   5:29198763-29203128question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000160888 ENSMUST00000001247 ENSMUST00000161398 ENSMUST00000160246 ENSMUST00000162975 ENSMUST00000168460

Gene Information

Description Not Available

Gene Symbol:   Rnf32
Gene Name:   Ring finger protein 32
Aliases:   1700009J01Rik, 2700025B22Rik, 4930542N22Rik, Lmbr2
RefSeq:   NC_000071.6 NT_165760.3
Ensembl:   ENSMUSG00000029130
Entrez:   56874
UniGene:   Mm.87352
Chromosome Mapping:   5 B1|5 15.81 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.010000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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