PrimePCR™ SYBR® Green Assay: Slc45a1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0037365

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0027311
Assay Design:   Intron-spanning
Chromosome Location:   4:150642599-150643985question
Amplicon Length:   173
Splice Variants Targeted:   ENSMUST00000037827 ENSMUST00000117997

Gene Information

Description Not Available

Gene Symbol:   Slc45a1
Gene Name:   Solute carrier family 45, member 1
Aliases:   C230078B22, Dnb5
RefSeq:   NC_000070.6 NT_187025.1 NT_187033.1
Ensembl:   ENSMUSG00000039838
Entrez:   242773
UniGene:   Mm.29280
Chromosome Mapping:   4 E2|4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.770000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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