PrimePCR™ SYBR® Green Assay: Lck, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0037300

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0027225
Assay Design:   Intron-spanning
Chromosome Location:   4:129551976-129555705question
Amplicon Length:   186
Splice Variants Targeted:   ENSMUST00000067240 ENSMUST00000102596 ENSMUST00000167288

Gene Information

Description Not Available

Gene Symbol:   Lck
Gene Name:   Lymphocyte protein tyrosine kinase
Aliases:   Hck-3, Lsk, Lskt, p56, p56Lck
RefSeq:   NC_000070.6 NT_039264.7 NT_187023.1
Ensembl:   ENSMUSG00000000409
Entrez:   16818
UniGene:   Mm.293753
Chromosome Mapping:   4 D2.2|4 59.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.020000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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