PrimePCR™ SYBR® Green Assay: Mrvi1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Mrvi1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Mrvi1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0025712
Assay Design:   Intron-spanning
Chromosome Location:   7:110876895-110878189question
Amplicon Length:   100
Splice Variants Targeted:   ENSMUST00000005751 ENSMUST00000125758 ENSMUST00000127935

Gene Information

This gene is a putative tumor suppressor gene that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein which is found in the membrane of the endoplasmic reticulum is similar to Jaw1 a lymphoid-restricted protein whose expression is downregulated during myeloid differentiation. Studies in mouse suggest that MRV integration at this locus induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Additional isoforms may arise through the use of alternative non-AUG (CUG) translation initiation site. [provided by RefSeq Jul 2008]

Gene Symbol:   Mrvi1
Gene Name:   MRV integration site 1
Aliases:   BB115629, Irag, Ris1
RefSeq:   NC_000073.6 NT_039433.8
Ensembl:   ENSMUSG00000005611
Entrez:   17540
Chromosome Mapping:   7 F1|7 51.52 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.100000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download