This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: Mdfic, Mouse
PrimePCR™ Template for SYBR® Green Assay: Mdfic, Mouse
This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG) and an in-frame downstream AUG codon results in the production of two isoforms which in human have been shown to have different subcellular localization. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.