PrimePCR™ SYBR® Green Assay: Mknk1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0025306
Assay Design:   Intron-spanning
Chromosome Location:   4:115857061-115860116question
Amplicon Length:   140
Splice Variants Targeted:   ENSMUST00000130819 ENSMUST00000144427 ENSMUST00000019677 ENSMUST00000106513 ENSMUST00000151203

Gene Information

Description Not Available

Gene Symbol:   Mknk1
Gene Name:   MAP kinase-interacting serine/threonine kinase 1
Aliases:   2410048M24Rik, Mnk1
RefSeq:   NC_000070.6 NT_039264.7
Ensembl:   ENSMUSG00000028708
Entrez:   17346
UniGene:   Mm.209327
Chromosome Mapping:   4 D1|4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.800000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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