PrimePCR™ SYBR® Green Assay: A4galt, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0036538

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024874
Assay Design:   Intron-spanning
Chromosome Location:   15:83228516-83230601question
Amplicon Length:   137
Splice Variants Targeted:   ENSMUST00000049530 ENSMUST00000164614

Gene Information

The protein encoded by this gene catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide which has been identified as the P(k) antigen of the P blood group system. The encoded protein which is a type II membrane protein found in the Golgi is also required for the synthesis of the bacterial verotoxins receptor. Alternative splicing results in multiple transcript variants.[provided by RefSeq Jan 2010]

Gene Symbol:   A4galt
Gene Name:   Alpha 1,4-galactosyltransferase
Aliases:   Not Available
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000047878
Entrez:   239559
Chromosome Mapping:   15 E1|15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.670000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download