PrimePCR™ SYBR® Green Assay: Rab11fip5, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Rab11fip5, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Rab11fip5, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024400
Assay Design:   Intron-spanning
Chromosome Location:   6:85348963-85374213question
Amplicon Length:   115
Splice Variants Targeted:   ENSMUST00000109124 ENSMUST00000164280 ENSMUST00000102766 ENSMUST00000109122 ENSMUST00000109119 ENSMUST00000020628 ENSMUST00000042084 ENSMUST00000161639 ENSMUST00000161913 ENSMUST00000161825 ENSMUST00000022170 ENSMUST00000060837 ENSMUST00000039892 ENSMUST00000172020 ENSMUST00000105359

Gene Information

Description Not Available

Gene Symbol:   Rab11fip5
Gene Name:   RAB11 family interacting protein 5 (class I)
Aliases:   9130206P09Rik, C75969, D6Ertd32e, GAF1, RIP11, mKIAA0857
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000051343
Entrez:   52055
Chromosome Mapping:   6 C3|6 35.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 34.900000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download