PrimePCR™ SYBR® Green Assay: Tnnc2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024397
Assay Design:   Intron-spanning
Chromosome Location:   2:164778176-164779716question
Amplicon Length:   76
Splice Variants Targeted:   ENSMUST00000004959 ENSMUST00000031879 ENSMUST00000103095 ENSMUST00000164539 ENSMUST00000066532 ENSMUST00000156290 ENSMUST00000040111 ENSMUST00000145963 ENSMUST00000096318 ENSMUST00000121484 ENSMUST00000106157 ENSMUST00000036328 ENSMUST00000036467 ENSMUST00000061506 ENSMUST00000000033 ENSMUST00000121128 ENSMUST00000105936 ENSMUST00000097936 ENSMUST00000102491 ENSMUST00000097816 ENSMUST00000168157 ENSMUST00000040222 ENSMUST00000004134 ENSMUST00000023312 ENSMUST00000167115 ENSMUST00000170035 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Tnnc2
Gene Name:   Troponin C2, fast
Aliases:   Tncs
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000017300
Entrez:   21925
UniGene:   Mm.1716
Chromosome Mapping:   2 H3|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 37.130000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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