PrimePCR™ SYBR® Green Assay: Tysnd1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0036410

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Tysnd1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Tysnd1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024393
Assay Design:   Intron-spanning
Chromosome Location:   10:61699516-61702149question
Amplicon Length:   199
Splice Variants Targeted:   ENSMUST00000020284 ENSMUST00000021794 ENSMUST00000163623 ENSMUST00000165665 ENSMUST00000072767 ENSMUST00000162792 ENSMUST00000040603

Gene Information

This gene encodes a protease that removes the N-terminal peroxisomal targeting signal (PTS2) from proteins produced in the cytosol thereby facilitating their import into the peroxisome. The encoded protein is also capable of removing the C-terminal peroxisomal targeting signal (PTS1) from proteins in the peroxisomal matrix. The full-length protein undergoes self-cleavage to produce shorter potentially inactive peptides. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq Jan 2013]

Gene Symbol:   Tysnd1
Gene Name:   Trypsin domain containing 1
Aliases:   1300019N10Rik
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000020087
Entrez:   71767
Chromosome Mapping:   10 B4|10

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.690000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download