PrimePCR™ SYBR® Green Assay: Zfyve21, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Zfyve21, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Zfyve21, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024376
Assay Design:   Intron-spanning
Chromosome Location:   12:111823756-111824913question
Amplicon Length:   199
Splice Variants Targeted:   ENSMUST00000051209 ENSMUST00000023828 ENSMUST00000135383 ENSMUST00000021714 ENSMUST00000000219 ENSMUST00000105929 ENSMUST00000066148 ENSMUST00000125995 ENSMUST00000003554 ENSMUST00000018549 ENSMUST00000108303 ENSMUST00000036156 ENSMUST00000168176 ENSMUST00000067543 ENSMUST00000034945 ENSMUST00000060253 ENSMUST00000113734 ENSMUST00000063605 ENSMUST00000043150 ENSMUST00000049393

Gene Information

Description Not Available

Gene Symbol:   Zfyve21
Gene Name:   Zinc finger, FYVE domain containing 21
Aliases:   1110013H04Rik, AW558780, C85416
RefSeq:   NC_000078.6 NT_039551.8
Ensembl:   ENSMUSG00000021286
Entrez:   68520
Chromosome Mapping:   12 F1|12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.880000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download