PrimePCR™ SYBR® Green Assay: Mrm1, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024371
Assay Design:   Intron-spanning
Chromosome Location:   11:84814936-84818647question
Amplicon Length:   123
Splice Variants Targeted:   ENSMUST00000048263 ENSMUST00000090027 ENSMUST00000169910 ENSMUST00000121990 ENSMUST00000078095 ENSMUST00000032338 ENSMUST00000056946 ENSMUST00000163226 ENSMUST00000018549 ENSMUST00000106763 ENSMUST00000106762 ENSMUST00000057892 ENSMUST00000119224 ENSMUST00000047664

Gene Information

Description Not Available

Gene Symbol:   Mrm1
Gene Name:   Mitochondrial rRNA methyltransferase 1 homolog (S. cerevisiae)
Aliases:   A530065E19Rik, ENSMUSG00000054212
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000018405
Entrez:   217038
UniGene:   Mm.280825
Chromosome Mapping:   11 C|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.890000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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