PrimePCR™ SYBR® Green Assay: Aqp3, Mouse

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024368
Assay Design:   Intron-spanning
Chromosome Location:   4:41095151-41098110question
Amplicon Length:   200
Splice Variants Targeted:   ENSMUST00000082125 ENSMUST00000072177 ENSMUST00000006669 ENSMUST00000075515 ENSMUST00000114299 ENSMUST00000033699 ENSMUST00000130007 ENSMUST00000101454 ENSMUST00000129308 ENSMUST00000055327 ENSMUST00000080449 ENSMUST00000019577

Gene Information

Description Not Available

Gene Symbol:   Aqp3
Gene Name:   Aquaporin 3
Aliases:   AQP-2
RefSeq:   NC_000070.6 NT_187032.1
Ensembl:   ENSMUSG00000028435
Entrez:   11828
UniGene:   Mm.34043
Chromosome Mapping:   4 B1|4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.160000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.