PrimePCR™ SYBR® Green Assay: Gnmt, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Gnmt, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Gnmt, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024353
Assay Design:   Intron-spanning
Chromosome Location:   17:46727331-46729102question
Amplicon Length:   189
Splice Variants Targeted:   ENSMUST00000111820 ENSMUST00000140224 ENSMUST00000082130 ENSMUST00000028759 ENSMUST00000053364 ENSMUST00000077066 ENSMUST00000145963 ENSMUST00000106224 ENSMUST00000097897 ENSMUST00000084301 ENSMUST00000170188 ENSMUST00000085116 ENSMUST00000112925 ENSMUST00000112923 ENSMUST00000038234 ENSMUST00000002846 ENSMUST00000149146 ENSMUST00000050555 ENSMUST00000103224 ENSMUST00000028843 ENSMUST00000154396 ENSMUST00000074875 ENSMUST00000092821 ENSMUST00000038388 ENSMUST00000026236 ENSMUST00000099492 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Gnmt
Gene Name:   Glycine N-methyltransferase
Aliases:   Not Available
RefSeq:   NC_000083.6 NT_039649.8
Ensembl:   ENSMUSG00000002769
Entrez:   14711
Chromosome Mapping:   17 C|17 10.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.160000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download