PrimePCR™ SYBR® Green Assay: Cyp2w1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024348
Assay Design:   Intron-spanning
Chromosome Location:   5:139354547-139355665question
Amplicon Length:   153
Splice Variants Targeted:   ENSMUST00000090813 ENSMUST00000036473 ENSMUST00000179619 ENSMUST00000093271 ENSMUST00000073337 ENSMUST00000143074 ENSMUST00000031521

Gene Information

Description Not Available

Gene Symbol:   Cyp2w1
Gene Name:   Cytochrome P450, family 2, subfamily w, polypeptide 1
Aliases:   Gm455
RefSeq:   NC_000071.6 NT_039316.8
Ensembl:   ENSMUSG00000029541
Entrez:   545817
UniGene:   Mm.482474
Chromosome Mapping:   5 G2|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.780000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download