PrimePCR™ SYBR® Green Assay: Ube2d3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024305
Assay Design:   Intron-spanning
Chromosome Location:   3:135438330-135439714question
Amplicon Length:   64
Splice Variants Targeted:   ENSMUST00000026274 ENSMUST00000024702 ENSMUST00000010210 ENSMUST00000168532 ENSMUST00000085092 ENSMUST00000164988 ENSMUST00000035824 ENSMUST00000106291

Gene Information

Description Not Available

Gene Symbol:   Ube2d3
Gene Name:   Ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)
Aliases:   1100001F19Rik, 9430029A22Rik, AA414951
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000078578
Entrez:   66105
UniGene:   Mm.49884
Chromosome Mapping:   3 G3|3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.590000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download