PrimePCR™ SYBR® Green Assay: Yjefn3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024304
Assay Design:   Intron-spanning
Chromosome Location:   8:69889503-69890936question
Amplicon Length:   92
Splice Variants Targeted:   ENSMUST00000031017 ENSMUST00000003207 ENSMUST00000054301 ENSMUST00000149349 ENSMUST00000051117 ENSMUST00000125149 ENSMUST00000027384 ENSMUST00000164538 ENSMUST00000016172 ENSMUST00000152938 ENSMUST00000023464 ENSMUST00000171172 ENSMUST00000110042 ENSMUST00000007250 ENSMUST00000097338 ENSMUST00000174026 ENSMUST00000070699 ENSMUST00000102852 ENSMUST00000160583 ENSMUST00000019076 ENSMUST00000067512

Gene Information

Description Not Available

Gene Symbol:   Yjefn3
Gene Name:   YjeF N-terminal domain containing 3
Aliases:   Gm175
RefSeq:   NC_000074.6 NT_039462.8
Ensembl:   ENSMUSG00000048967
Entrez:   234365
UniGene:   Mm.252521
Chromosome Mapping:   8 B3.3|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.980000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download