PrimePCR™ SYBR® Green Assay: Kcnk16, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024296
Assay Design:   Intron-spanning
Chromosome Location:   14:20265254-20269072question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000106113 ENSMUST00000106830 ENSMUST00000062474 ENSMUST00000085555 ENSMUST00000082209 ENSMUST00000108910 ENSMUST00000108909 ENSMUST00000108908 ENSMUST00000049378 ENSMUST00000166783 ENSMUST00000051720 ENSMUST00000110515 ENSMUST00000071878 ENSMUST00000110514 ENSMUST00000068004 ENSMUST00000113825 ENSMUST00000055485 ENSMUST00000058764 ENSMUST00000106259 ENSMUST00000026666 ENSMUST00000161522 ENSMUST00000159975 ENSMUST00000032811 ENSMUST00000094617 ENSMUST00000068792 ENSMUST00000022213 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Kcnk16
Gene Name:   Potassium channel, subfamily K, member 16
Aliases:   4731413G05Rik, TALK-1, TALK1
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000023387
Entrez:   74571
UniGene:   Mm.105571
Chromosome Mapping:   14 B|14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.630000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download