PrimePCR™ SYBR® Green Assay: Nlrp6, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Nlrp6, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Nlrp6, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024291
Assay Design:   Intron-spanning
Chromosome Location:   7:140924129-140925185question
Amplicon Length:   107
Splice Variants Targeted:   ENSMUST00000122912 ENSMUST00000053910 ENSMUST00000176109 ENSMUST00000145598 ENSMUST00000112394 ENSMUST00000041627 ENSMUST00000023776 ENSMUST00000162049 ENSMUST00000007318 ENSMUST00000126153 ENSMUST00000137498 ENSMUST00000026486 ENSMUST00000119746 ENSMUST00000169582 ENSMUST00000059906 ENSMUST00000115444 ENSMUST00000069623 ENSMUST00000097820 ENSMUST00000105799 ENSMUST00000105798 ENSMUST00000105797 ENSMUST00000039204 ENSMUST00000002081 ENSMUST00000106045 ENSMUST00000106709 ENSMUST00000106708 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Nlrp6
Gene Name:   NLR family, pyrin domain containing 6
Aliases:   AI504961, Nalp6, Navr/Avr, Pypaf5
RefSeq:   NC_000073.6 NT_166306.2
Ensembl:   ENSMUSG00000038745
Entrez:   101613
Chromosome Mapping:   7 F5|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.430000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download