PrimePCR™ SYBR® Green Assay: Slc25a25, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0036031

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0023937
Assay Design:   Intron-spanning
Chromosome Location:   2:32419048-32420363question
Amplicon Length:   188
Splice Variants Targeted:   ENSMUST00000028160 ENSMUST00000052119 ENSMUST00000113310 ENSMUST00000113308 ENSMUST00000113307 ENSMUST00000136361

Gene Information

Description Not Available

Gene Symbol:   Slc25a25
Gene Name:   Solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 25
Aliases:   1110030N17Rik, MCSC, mKIAA1896
RefSeq:   NC_000068.7 NT_039206.8
Ensembl:   ENSMUSG00000026819
Entrez:   227731
UniGene:   Mm.37395
Chromosome Mapping:   2 B|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.750000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download