PrimePCR™ SYBR® Green Assay: Mras, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0035731

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0023547
Assay Design:   Intron-spanning
Chromosome Location:   9:99394515-99411494question
Amplicon Length:   180
Splice Variants Targeted:   ENSMUST00000035045 ENSMUST00000122384 ENSMUST00000119472 ENSMUST00000123771

Gene Information

Description Not Available

Gene Symbol:   Mras
Gene Name:   Muscle and microspikes RAS
Aliases:   2900078C09Rik, AI326250
RefSeq:   NC_000075.6 NT_039474.8
Ensembl:   ENSMUSG00000032470
Entrez:   17532
UniGene:   Mm.2045
Chromosome Mapping:   9 E3.3|9

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.950000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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