PrimePCR™ SYBR® Green Assay: Obscn, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0034340

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0022299
Assay Design:   Intron-spanning
Chromosome Location:   11:59022307-59027470question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000138587 ENSMUST00000020732 ENSMUST00000127937 ENSMUST00000047441 ENSMUST00000081658 ENSMUST00000052872

Gene Information

The obscurin gene spans more than 150 kb contains over 80 exons and encodes a protein of approximately 800 kDa. The encoded protein contains 68 Ig domains 2 fibronectin domains 1 calcium/calmodulin-binding domain 1 RhoGEF domain with an associated PH domain and 2 serine-threonine kinase domains. This protein is one of three giant sacromeric signaling proteins that includes titin and nebulin. It may have a role in the organization of myofibrils during assembly and also may mediate interactions between the sarcoplasmic reticulum and myofibrils. Alternatively spliced transcript variants encoding different isoforms have been described although the full-length nature is not known for all splicing variants. [provided by RefSeq Jan 2010]

Gene Symbol:   Obscn
Gene Name:   Obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Aliases:   BC046431, Gm878, OTTMUSG00000005786, UNC89
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000061462
Entrez:   380698
UniGene:   Mm.339542
Chromosome Mapping:   11 B1.3|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.290000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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