This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCIP0034340
PrimePCR™ PreAmp for SYBR® Green Assay: Obscn, Mouse
PrimePCR™ Template for SYBR® Green Assay: Obscn, Mouse
The obscurin gene spans more than 150 kb contains over 80 exons and encodes a protein of approximately 800 kDa. The encoded protein contains 68 Ig domains 2 fibronectin domains 1 calcium/calmodulin-binding domain 1 RhoGEF domain with an associated PH domain and 2 serine-threonine kinase domains. This protein is one of three giant sacromeric signaling proteins that includes titin and nebulin. It may have a role in the organization of myofibrils during assembly and also may mediate interactions between the sarcoplasmic reticulum and myofibrils. Alternatively spliced transcript variants encoding different isoforms have been described although the full-length nature is not known for all splicing variants. [provided by RefSeq Jan 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.