This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCIP0034210
PrimePCR™ PreAmp for SYBR® Green Assay: Trim24, Mouse
PrimePCR™ Template for SYBR® Green Assay: Trim24, Mouse
The protein encoded by this gene is part of the tripartite-motif containing family (TRIM) which are typified by the RING B-box type 1 B-box type 2 and coiled-coil region domains. This protein which also contains a PHD/TTC finger and bromodomain important for regulating nuclear receptors and binding chromatin has important roles in differentiation development and tissue homeostasis. This protein has been reported to regulate the activity of the tumor suppressor p53 and of the retinoic acid receptor. A translocation event between this gene and Braf transforming gene which results in the fusion protein T18 has been reported in hepatocellular carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq Jan 2013]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.