PrimePCR™ SYBR® Green Assay: Snrnp200, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0021962
Assay Design:   Intron-spanning
Chromosome Location:   2:127225135-127226091question
Amplicon Length:   115
Splice Variants Targeted:   ENSMUST00000103220

Gene Information

On February 19 2002 this locus was switched from human to mouse. The source accession Z70200.1 is almost identical to the mouse BAC clone AC074224 and it matches the mouse cDNA accession BC011390 as well. The human gene is LocusID 23020. [provided by RefSeq Jul 2008]

Gene Symbol:   Snrnp200
Gene Name:   Small nuclear ribonucleoprotein 200 (U5)
Aliases:   A330064G03Rik, Ascc3l1, BC011390, HELIC2, U5-200-KD, U5-200KD
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000003660
Entrez:   320632
UniGene:   Mm.215860
Chromosome Mapping:   2 F1|2 62.2 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 34.780000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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