This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: Sep15, Mouse
PrimePCR™ Template for SYBR® Green Assay: Sep15, Mouse
This gene encodes a selenoprotein which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure the sec insertion sequence (SECIS) that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. The exact function of this protein is not known however it has been found to associate with UDP-glucose:glycoprotein glucosyltransferase (UGTR) an endoplasmic reticulum(ER)-resident protein which is involved in the quality control of protein folding. The association with UGTR retains this selenoprotein in the ER where it may play a role in protein folding. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.