This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: Stk10, Mouse
PrimePCR™ Template for SYBR® Green Assay: Stk10, Mouse
This gene encodes a member of the Ste20 family of serine/threonine protein kinases and is similar to several known polo-like kinase kinases. Mice deficient for this gene product are viable but exhibit altered integrin-mediated lymphocyte adhesion characteristics. The orthologous gene product in humans can associate with and phosphorylate polo-like kinase 1 and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.