PrimePCR™ SYBR® Green Assay: Chrm4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0021069
Assay Design:   Intron-spanning
Chromosome Location:   2:91922223-91927274question
Amplicon Length:   149
Splice Variants Targeted:   ENSMUST00000114571 ENSMUST00000077093 ENSMUST00000114572 ENSMUST00000074466 ENSMUST00000165519 ENSMUST00000035027 ENSMUST00000162295 ENSMUST00000087050 ENSMUST00000004143 ENSMUST00000107358 ENSMUST00000047521 ENSMUST00000134152 ENSMUST00000170432 ENSMUST00000094639 ENSMUST00000110639 ENSMUST00000113309 ENSMUST00000027434 ENSMUST00000131412 ENSMUST00000084535 ENSMUST00000031668 ENSMUST00000078912 ENSMUST00000114001 ENSMUST00000113999 ENSMUST00000064797 ENSMUST00000114002 ENSMUST00000056482 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Chrm4
Gene Name:   Cholinergic receptor, muscarinic 4
Aliases:   Chrm-4, M4
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000040495
Entrez:   12672
UniGene:   Mm.330405
Chromosome Mapping:   2 E1|2 49.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.987800
y-intercept 39.930000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download