PrimePCR™ SYBR® Green Assay: Ptgir, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0021065
Assay Design:   Intron-spanning
Chromosome Location:   7:16907474-16908784question
Amplicon Length:   199
Splice Variants Targeted:   ENSMUST00000144408 ENSMUST00000002436 ENSMUST00000007046 ENSMUST00000043259 ENSMUST00000106307 ENSMUST00000166542 ENSMUST00000087200 ENSMUST00000074372 ENSMUST00000101029 ENSMUST00000093785 ENSMUST00000087497 ENSMUST00000114255 ENSMUST00000114252 ENSMUST00000094339 ENSMUST00000166431 ENSMUST00000098441 ENSMUST00000159692 ENSMUST00000071898 ENSMUST00000107615 ENSMUST00000118375 ENSMUST00000107614 ENSMUST00000066078

Gene Information

Description Not Available

Gene Symbol:   Ptgir
Gene Name:   Prostaglandin I receptor (IP)
Aliases:   IP, PGI2
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000043017
Entrez:   19222
UniGene:   Mm.287572
Chromosome Mapping:   7 A2|7 2.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.580000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download