PrimePCR™ SYBR® Green Assay: Fstl3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0021049
Assay Design:   Intron-spanning
Chromosome Location:   10:79778590-79779999question
Amplicon Length:   164
Splice Variants Targeted:   ENSMUST00000090219 ENSMUST00000102613 ENSMUST00000060441 ENSMUST00000113808 ENSMUST00000022220 ENSMUST00000171263 ENSMUST00000045097 ENSMUST00000110627 ENSMUST00000110628 ENSMUST00000052029 ENSMUST00000110626 ENSMUST00000110625 ENSMUST00000009099 ENSMUST00000109723 ENSMUST00000069690 ENSMUST00000109726 ENSMUST00000109727 ENSMUST00000061552 ENSMUST00000074570 ENSMUST00000166802 ENSMUST00000034316 ENSMUST00000035766 ENSMUST00000101670 ENSMUST00000025617 ENSMUST00000020575 ENSMUST00000085222 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Fstl3
Gene Name:   Follistatin-like 3
Aliases:   E030038F23Rik, Flrg
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000020325
Entrez:   83554
UniGene:   Mm.490276
Chromosome Mapping:   10 C1|10

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.610000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download