PrimePCR™ SYBR® Green Assay: Tubb6, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0021013
Assay Design:   Intron-spanning
Chromosome Location:   18:67393015-67401398question
Amplicon Length:   101
Splice Variants Targeted:   ENSMUST00000001513 ENSMUST00000179460 ENSMUST00000043584 ENSMUST00000072451 ENSMUST00000115393 ENSMUST00000050859 ENSMUST00000167746 ENSMUST00000110391

Gene Information

Description Not Available

Gene Symbol:   Tubb6
Gene Name:   Tubulin, beta 6 class V
Aliases:   2310057H16Rik, BB220206
RefSeq:   NC_000084.6 NT_039674.8
Ensembl:   ENSMUSG00000001473
Entrez:   67951
UniGene:   Mm.181860
Chromosome Mapping:   18|18 E1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.700000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download