PrimePCR™ SYBR® Green Assay: Myl6, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Myl6, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Myl6, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0021010
Assay Design:   Intron-spanning
Chromosome Location:   10:128492685-128493850question
Amplicon Length:   101
Splice Variants Targeted:   ENSMUST00000030187 ENSMUST00000088653 ENSMUST00000164181 ENSMUST00000048112 ENSMUST00000057209 ENSMUST00000042717 ENSMUST00000016124 ENSMUST00000106104 ENSMUST00000001667 ENSMUST00000113267 ENSMUST00000067924 ENSMUST00000168438 ENSMUST00000090750 ENSMUST00000107038 ENSMUST00000040357 ENSMUST00000097474 ENSMUST00000055539 ENSMUST00000105244 ENSMUST00000105242 ENSMUST00000105245 ENSMUST00000046875

Gene Information

Description Not Available

Gene Symbol:   Myl6
Gene Name:   Myosin, light polypeptide 6, alkali, smooth muscle and non-muscle
Aliases:   ESMLC, LC17, LC17-GI, LC17-NM, LC17A, LC17B, MLC-3, MLC1SM, MLC3SM, MLC3nm, Myln
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000090841
Entrez:   17904
Chromosome Mapping:   10 D3|10

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.750000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download